Amplification of near full-length HIV-1 proviruses for next-generation sequencing
The Full-Length Individual Proviral Sequencing (FLIPS) assay is an efficient and high-
throughput method designed to amplify and sequence single, near full-length (intact and
defective), HIV-1 proviruses. FLIPS allows determination of the genetic composition of
integrated HIV-1 within a cell population. Through identifying defects within HIV-1 proviral
sequences that arise during reverse transcription, such as large internal deletions,
deleterious stop codons/hypermutation, frameshift mutations, and mutations/deletions in cis …
throughput method designed to amplify and sequence single, near full-length (intact and
defective), HIV-1 proviruses. FLIPS allows determination of the genetic composition of
integrated HIV-1 within a cell population. Through identifying defects within HIV-1 proviral
sequences that arise during reverse transcription, such as large internal deletions,
deleterious stop codons/hypermutation, frameshift mutations, and mutations/deletions in cis …