[HTML][HTML] Identification of CDK4 sequences involved in cyclin D1 and p16 binding
KG Coleman, BS Wautlet, D Morrissey… - Journal of biological …, 1997 - ASBMB
Activation of CDK4 is regulated, in part, by its association with a D-type cyclin. Conversely,
CDK4 activity is inhibited when it is bound to the cyclin-dependent kinase inhibitor, p16
INK4A. To investigate the molecular basis of the interactions between CDK4 and cyclin D1
or p16 INK4A we performed site-directed mutagenesis of CDK4. The interaction was
examined using in vitro translated wild type and mutant CDK4 proteins and bacterially
expressed cyclin D1 and p16 fusion proteins. As mutational analysis of CDC2 suggests that …
CDK4 activity is inhibited when it is bound to the cyclin-dependent kinase inhibitor, p16
INK4A. To investigate the molecular basis of the interactions between CDK4 and cyclin D1
or p16 INK4A we performed site-directed mutagenesis of CDK4. The interaction was
examined using in vitro translated wild type and mutant CDK4 proteins and bacterially
expressed cyclin D1 and p16 fusion proteins. As mutational analysis of CDC2 suggests that …