Culture of neonatal rodent microglia, astrocytes, and oligodendrocytes from the cortex, spinal cord, and cerebellum
SD Skaper, L Facci - Neurotrophic Factors: Methods and Protocols, 2018 - Springer
SD Skaper, L Facci
Neurotrophic Factors: Methods and Protocols, 2018•SpringerThe protocol described in this chapter covers the preparation and culture of enriched
populations of microglia, astrocytes, and oligodendrocytes from the cortex and spinal cord of
neonatal rat and mouse. The procedure is based on enzymatic digestion of the tissue,
followed by the culture of a mixed glial cell population which is then utilized as the starting
point for the isolation, via differential attachment, of the different cell types.
populations of microglia, astrocytes, and oligodendrocytes from the cortex and spinal cord of
neonatal rat and mouse. The procedure is based on enzymatic digestion of the tissue,
followed by the culture of a mixed glial cell population which is then utilized as the starting
point for the isolation, via differential attachment, of the different cell types.
Abstract
The protocol described in this chapter covers the preparation and culture of enriched populations of microglia, astrocytes, and oligodendrocytes from the cortex and spinal cord of neonatal rat and mouse. The procedure is based on enzymatic digestion of the tissue, followed by the culture of a mixed glial cell population which is then utilized as the starting point for the isolation, via differential attachment, of the different cell types.
Springer
