[HTML][HTML] SphK1 and SphK2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolism
M Maceyka, H Sankala, NC Hait, H Le Stunff… - Journal of Biological …, 2005 - ASBMB
The potent sphingolipid metabolite sphingosine 1-phosphate is produced by
phosphorylation of sphingosine catalyzed by sphingosine kinase (SphK) types 1 and 2. In
contrast to pro-survival SphK1, the putative BH3-only protein SphK2 inhibits cell growth and
enhances apoptosis. Here we show that SphK2 catalytic activity also contributes to its ability
to induce apoptosis. Overexpressed SphK2 also increased cytosolic free calcium induced by
serum starvation. Transfer of calcium to mitochondria was required for SphK2-induced …
phosphorylation of sphingosine catalyzed by sphingosine kinase (SphK) types 1 and 2. In
contrast to pro-survival SphK1, the putative BH3-only protein SphK2 inhibits cell growth and
enhances apoptosis. Here we show that SphK2 catalytic activity also contributes to its ability
to induce apoptosis. Overexpressed SphK2 also increased cytosolic free calcium induced by
serum starvation. Transfer of calcium to mitochondria was required for SphK2-induced …
