Coding potential of laboratory and clinical strains of human cytomegalovirus
E Murphy, D Yu, J Grimwood… - Proceedings of the …, 2003 - National Acad Sciences
Proceedings of the National Academy of Sciences, 2003•National Acad Sciences
Six strains of human cytomegalovirus have been sequenced, including two laboratory
strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four
clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX,
PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial
artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been
identified that are conserved in all four clinical isolates of the virus. Multiple sequence …
strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four
clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX,
PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial
artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been
identified that are conserved in all four clinical isolates of the virus. Multiple sequence …
Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.
National Acad Sciences