Cholesterol efflux from cultured adipose cells is mediated by LpAI particles but not by LpAI: AII particles
R Barbaras, P Puchois, JC Fruchart… - … and Biophysical Research …, 1987 - Elsevier
R Barbaras, P Puchois, JC Fruchart, G Ailhaud
Biochemical and Biophysical Research Communications, 1987•ElsevierCholesterol efflux was studied in cultured adipose cells after preloading with LDL
cholesterol. Long-term exposure to LpA I and LpA I: A II particles isolated from the HDL
fraction showed that LpA I particles only were able to promote cholesterol efflux. Liposomes
containing different ApoA I ApoA II molar ratios were tested: the larger the proportion of
ApoA I, the faster the ability to remove cholesterol from Ob1771 cells. Dose-response curves
showed that LpA I particles were active within a physiological range of concentrations …
cholesterol. Long-term exposure to LpA I and LpA I: A II particles isolated from the HDL
fraction showed that LpA I particles only were able to promote cholesterol efflux. Liposomes
containing different ApoA I ApoA II molar ratios were tested: the larger the proportion of
ApoA I, the faster the ability to remove cholesterol from Ob1771 cells. Dose-response curves
showed that LpA I particles were active within a physiological range of concentrations …
Cholesterol efflux was studied in cultured adipose cells after preloading with LDL cholesterol. Long-term exposure to LpA I and LpA I: A II particles isolated from the HDL fraction showed that LpA I particles only were able to promote cholesterol efflux. Liposomes containing different ApoA I ApoA II molar ratios were tested: the larger the proportion of ApoA I, the faster the ability to remove cholesterol from Ob1771 cells. Dose-response curves showed that LpA I particles were active within a physiological range of concentrations, whereas LpA I: A II particles had no effect at all concentrations. The results are in favour of LpA I particles being the active components of the HDL fraction for the promotion of cholesterol efflux and suggest that LpA I particles and LpA I: A II particles represent distinct metabolic entities.
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